Advanced technology
- Special fluorescent staining dyes DNA/RNA, while 2nd-generation chemistry staining reagents dye enzymes and particles in the cytoplasm
- The more DNA or RNA in a cell, the stronger the fluorescent signal, so abnormal cells are distinguished with higher sensitivity
- Flow cytometry: every cell is detected by three beams of light from three directions
- FSL (forward scattered light) reflects cell size; SSC (side scattered light) reflects size and number of particles; SFL (side fluorescence light) reflects nucleic acid concentration



